بایل اسکولین آگار بیس 500 گرمی میکرومدیا مجارستان

Description of MM0136


 

Bile Esculin Agar

 

 

Use and description:  

For the isolation and presumptive identification of enterococci (Group D streptococci). Enterococci has the ability to hydrolyse esculin. The final product, dihydroxy-6,7 coumarin, combines with iron ions to produce a dark to black-coloured compound that diffuses into the medium (black halo arround the colonies). Bile renders the medium inhibitor with regard to other bacteria.

 

Composition per liter:

Bile salts………………………….20.0 g

Agar………………………………13.0 g

Peptone…………………………….5.0 g

Meat extract………………………..3.0 g

Ferric citrate……………………….0.5 g

Esculin……………………………..1.0 g

 

Final pH of the ready to use medium:   6,9 ± 0.2

 

Medium preparation:  

Add 42.5 grams of dehydrated culture medium to 1 liter of distilled water. Heat with repeated stirring to dissolve completely. Sterilise by autoclaving at 121oC for 15 minutes.

 

Quality specifications:    

Dehydrated medium: homogeneous, beige fine powder.

Ready to use medium: dark amber with blueish cast and opalescent.

 

Microbiological response:

 

 Organism

Result

Escherichia coli ATCC 25922

Growth, Esculin (-)

Enterococcus faecalis ATCC 29212

Growth, Esculin (+)

Streptococcus pyogenes ATCC 19615

Inhibited, Esculin (-)

Listeria monocytogenes ATCC 7644

Inhibited

 

Storage:  

Dehydrated medium should be stored between 10 to 25oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original.


فروشنده: آزمیران
گارانتی اصالت و سلامت کالا
پشتیبانی ۷ روز هفته ۲۴ ساعته
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