پپتون سویا ( سوی پپتون ) ساخت شرکت میکرومدیا مجارستان 500 گرمی

Description of MM0337

Raka Ray agar

 

Use and description:  

Medium used for the isolation of lactic acid bacteria in beer and brewing processes. It enables monitoring in-process beer quickly for numerous organisms.

 

Composition per liter:

 

Yeast extract………………………………….......5.00 g

Tryptone……………………………………….  20.00 g

Liver extract……………………………………...1.00 g

Maltose………………………………………….10.00 g

Fructose…………………………………………..5.00 g

Glucose…………………………………………...5.00 g

Betain HCl………………………………………..2.00 g

Diammonium-H-citrate…………………………..2.00 g

Potassium aspartame……………………………..2.50 g

Potassium glutamate……………………………...2.50 g

Magnesium sulphate……………………………...0.98 g

Manganese sulphate………………………………0.42 g

Potassium phosphate……………………………...2.00 g

N-acetyl-glucosamine…………………………….0.50 g

Agar……………………………………………..15.00 g

 

Final pH of the ready to use medium:   5.4 ± 0.2

 

Medium preparation:   Add 73.9 grams of dehydrated culture medium to 1 liter of distilled water and add 10 ml Tween 80 to it. Mix until evenly dispersed with stirring. Boil for one minute. Autoclave for 15 min at 121 °C and to increase selectivity add aseptically 3 mg cycloheximide and 3 g phenylethanol and pour into tubes or petri dishes.

 

Quality specifications:    

Dehydrated medium:  homogeneous, beige fine powder.

Ready to use medium:  clear to sligthly haisy,  amber colored agar.

 

Microbiological response after 24-48 hours at 27-30 °C, under anaerobic condition:

 

Organism                                                                             Result

E.coli ATCC 25922

inhibition or poor growth

Lactobacillus fermentans ATCC 9338

good growth

 

Storage:   Dehydrated medium should be stored between 10 to 25.0oC. Once opened, place the container in a dark, dry place. The dehydrated medium should not be used if there is any lump or the color has changed from the original.


فروشنده: آزمیران
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